Anti-PPHLN1/Periphilin-1 Rabbit pAb

Anti-PPHLN1/Periphilin-1 Rabbit pAb

Antibodies Oncology
AO-03-GB112022-50
PPHLN 1,CDC7 Expression Repressor,HSPC206,Periphilin 1,Pphln1,CR,PPHLN1/Periphilin-1,112022,Periphilin1,PPHLN-1,Gastric cancer antigen Ga50,GB112022,HSPC232,Anti- PPHLN1/Periphilin-1 Rabbit pAb,Periphilin-1
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Product Information


Protein full name

Periphilin-1

Synonyms

Pphln1, Gastric cancer antigen Ga50, CDC7 Expression Repressor , CR, HSPC206, HSPC232

Uniprot ID

Q8K2H1

Immunogen

Recombinant protein corresponding to Mouse PPHLN1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 500-1: 2000 brain, skin, testis



Background

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation.



Images

01pFKwyo65jMh8m.jpg

 IHC analysis of PPHLN1/Periphilin-1(GB112022).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02yQoIHVsVaq87R.jpg

 IF analysis of PPHLN1/Periphilin-1(GB112022)red.
Sample: Mouse skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 650, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

03Rm8PUpRgjvTU0.jpg

 IF analysis of PPHLN1/Periphilin-1(GB112022)red.
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 650, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04W1dUhpLbd0fis.jpg

 IF analysis of PPHLN1/Periphilin-1(GB112022)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 650, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112022-50
: 10 Items
Hurry! only 10 items left in stock.

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