Anti-CRIF1 Rabbit pAb

Anti-CRIF1 Rabbit pAb

Antibodies Oncology
AO-03-GB111592-50
G45IP_HUMAN,L2-interacting nuclear protein 1,p53 responsive gene 6,CRIF-1,Gadd45gip1,GB111592,111592,39S ribosomal protein L59,mitochondrial,CKbetaBP2,CKII beta binding protein 2,CRIF1,CRIF 1,Papillomavirus,MRP-L59,Growth arrest and DNA damage-inducible proteins-interacting protein 1
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Product Information


Protein full name

Growth arrest and DNA damage-inducible proteins-interacting protein 1

Synonyms

39S ribosomal protein L59, mitochondrial, MRP-L59, Gadd45gip1, CKbetaBP2, CKII beta binding protein 2, G45IP_HUMAN, p53 responsive gene 6, L2-interacting nuclear protein 1, Papillomavirus

Uniprot ID

Q9CR59

Immunogen

Recombinant protein corresponding to Mouse CRIF1

Isotype

IgG

Predicted MW./Observed MW.

26 kDa / 25 kDa

Purity

Affinity purification

Subcellular location

Mitochondrion, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 liver, spleen, lung
ICC/IF Human 1: 100-1: 400 HeLa



Background

GADD45GIP1 acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity.



Images

01dNvBCXDsDXdsA.jpg

 WB analysis of CRIF1(GB111592).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02foGjnxARSuJpJ.jpg

 ICC analysis of CRIF1(GB111592)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111592-50
: 10 Items
Hurry! only 10 items left in stock.

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