Anti-Rad6 Rabbit pAb

Anti-Rad6 Rabbit pAb

Antibodies Oncology
AO-03-GB111539-50
RAD6 homolog A,Anti-Rad6 Rabbit pAb,Ubiquitin-protein ligase A,GB111539100,111539,Ubiquitin carrier protein A,Rad6a,HR6A,mHR6A,Rad6,GB111539,Ube2a,E2 ubiquitin-conjugating enzyme A,Rad-6,Rad 6,Ubiquitin-conjugating enzyme E2 A
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Product Information


Protein full name

Ubiquitin-conjugating enzyme E2 A

Synonyms

E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, mHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, Ube2a, Rad6a

Uniprot ID

Q9Z255

Immunogen

Recombinant protein corresponding to Mouse Rad6

Isotype

IgG

Predicted MW./Observed MW.

17 kDa / 17 kDa

Purity

Affinity purification

Subcellular location

Late endosome, Lysosome



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 placenta, brain, kidney
ICC/IF Human 1: 300-1: 600 HeLa



Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions.



Images

01itnPVic6Pa23T.jpg

 WB analysis of Rad6(GB111539).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

028rcu9FmqS2QpV.jpg

 WB analysis of Rad6(GB111539).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03bPHaIUP5WFeWK.jpg

 WB analysis of Rad6(GB111539).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

044YcMIuxi6nRkH.jpg

 ICC analysis of Rad6(GB111539)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111539-50
: 10 Items
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