Anti-MSF Rabbit pAb

Anti-MSF Rabbit pAb

Antibodies Oncology
AO-03-GB111255-50
Ov/Br septin,SEPTIN9,SEPT9,Septin D1,Ovarian/Breast septin,MSF,MLL septin-like fusion protein MSF-A,GB111255,KIAA0991,111255,MLL septin-like fusion protein,Septin-9
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Product Information


Protein full name

Septin-9

Synonyms

MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein, Ovarian/Breast septin, Ov/Br septin, Septin D1, SEPTIN9, KIAA0991, MSF, SEPT9

Uniprot ID

Q80UG5

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MSF

Isotype

IgG

Predicted MW./Observed MW.

65 kDa / 75 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, cytoskeleton



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 900 SH-SY5Y, MDA-MB-231, DU145
ICC/IF Human, Mouse 1: 50-1: 200 HeLa, NIH/3T3



Background

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia.



Images

01g53NBoNPY6iTt.jpg

 WB analysis of MSF(GB111255).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02clwGgNoc1MgUE.jpg

 ICC analysis of MSF(GB111255)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 50, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

03w903UH287W4Uo.jpg

 ICC analysis of MSF(GB111255)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 50, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111255-50
: 10 Items
Hurry! only 10 items left in stock.

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