Product Information
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Protein full name |
Serine/threonine-protein kinase STK11 |
|
Synonyms |
Liver kinase B1 homolog, LKB1, mLKB1, Stk11, Lkb1 |
|
Uniprot ID |
Q9WTK7 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse LKB1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse, Rat | 1: 1000-1: 4000 | bone, brain, lung |
Background
Liver kinase B1 (LKB1), also known as serine/threonine-protein kinase STK11, is a member of the serine/threonine kinase family. LKB1 is a tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases. Regulates cell polarity by remodeling the actin cytoskeleton, regulates the inhibition of cell growth signaling pathways in response to low energy levels, regulates autophagy in response to nutrient deprivation, and is involved in DNA damage response by differentiating B-cells in the germinal center.
Images
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IHC analysis of LKB1(GB111236). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of LKB1(GB111236). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of LKB1(GB111236). Sample: Mouse lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of LKB1(GB111236). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of LKB1(GB111236). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of LKB1(GB111236). Sample: Rat testis, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IF analysis of LKB1(GB111236)red. Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
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IF analysis of LKB1(GB111236)red. Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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