Anti-Mad2L1 Rabbit pAb
   

Anti-Mad2L1 Rabbit pAb

Antibodies Oncology
AO-03-GB111185-50
111185,Mitotic arrest deficient 2-like protein 1,GB111185,MAD2-like protein 1,Mad2a,Mad 2L 1,Mad2L1,Mad-2L-1,Mitotic spindle assembly checkpoint protein MAD2A
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Product Information


Protein full name

Mitotic spindle assembly checkpoint protein MAD2A

Synonyms

Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, Mad2L1, Mad2a, HSMAD2, MAD2, MAD2 like protein 1, MAD2L1

Uniprot ID

Q9Z1B5

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Mad2L1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 500-1: 3000 heart, colon, ovary



Background

The Mad2L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.



Images

01YazR5XPj6nxyq.jpg

 IHC analysis of Mad2L1(GB111185).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02zlgamD556i8R0.jpg

 IHC analysis of Mad2L1(GB111185).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03ZY4dFJLniU90z.jpg

 IHC analysis of Mad2L1(GB111185).
Sample: Mouse ovary, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04VjelJrTj1080i.jpg

 IHC analysis of Mad2L1(GB111185).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

051ZIvHXcXJmWvE.jpg

 IHC analysis of Mad2L1(GB111185).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06r1x9L7aKHnfTo.jpg

 IHC analysis of Mad2L1(GB111185).
Sample: Rat ovary, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111185-50
: 10 Items
Hurry! only 10 items left in stock.

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