Anti-MDM2 Rabbit pAb
   

Anti-MDM2 Rabbit pAb

Antibodies Oncology
AO-03-GB111161-50
RING-type E3 ubiquitin transferase Mdm2,GB111161,p53-binding protein Mdm2,MDM 2,Double minute 2 protein,MDM2,Oncoprotein Mdm2,MDM-2,111161,E3 ubiquitin-protein ligase Mdm2
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Product Information


Protein full name

E3 ubiquitin-protein ligase Mdm2

Synonyms

Double minute 2 protein, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2, hdm2, HDMX, MDM2, p53 binding protein Mdm2

Uniprot ID

P23804

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MDM2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 1000-1: 3000 kidney, lung, thymus



Background

MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Over expression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.



Images

01zBVG05OD2XPhq.jpg

 IHC analysis of MDM2(GB111161).
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02QEydLTa86NJFM.jpg

 IHC analysis of MDM2(GB111161).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03ZBTUWh0tCI9F5.jpg

 IHC analysis of MDM2(GB111161).
Sample: Mouse thymus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04mfmETroh0DSAm.jpg

 IHC analysis of MDM2(GB111161).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05KpraKoFlh1wJF.jpg

 IHC analysis of MDM2(GB111161).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111161-50
: 10 Items
Hurry! only 10 items left in stock.

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