Anti-14-3-3 eta/YWHAH Rabbit pAb

Anti-14-3-3 eta/YWHAH Rabbit pAb

Antibodies Oncology
AO-03-GB11921-50
Ywhah,11921,GB11921,YWHA1,14 3 3,5-monooxygenase activation protein eta,1433,Tyrosine 3-monooxygenase/tryptophan,YWHAH,14-3-3 eta/YWHAH,14-3-3 η,14-3-3 protein eta
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Product Information


Protein full name

14-3-3 protein eta

Synonyms

Ywhah, YWHAH, YWHA1, Tyrosine 3-monooxygenase/tryptophan, 14-3-3 η, 5-monooxygenase activation protein eta

Uniprot ID

P68510

Immunogen

Recombinant protein corresponding to Mouse 14-3-3 η

Isotype

IgG

Predicted MW./Observed MW.

28 kDa / 30 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 brain, thymus, lung
IHC/IF Mouse, Rat 1: 500-1: 3000 skin



Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.



Images

01OkKcKYmqKRBvP.jpg

 WB analysis of 14-3-3 eta/YWHAH(GB11921).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02uEklvAUiRK769.jpg

 IHC analysis of 14-3-3 eta/YWHAH(GB11921).
Sample: Mouse skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03m6sLDHECU8ROi.jpg

 IHC analysis of 14-3-3 eta/YWHAH(GB11921).
Sample: Rat skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04uxLQyewhh9Vhk.jpg

 IF analysis of 14-3-3 eta/YWHAH(GB11921)red.
Sample: Rat skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11921-50
: 10 Items
Hurry! only 10 items left in stock.

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