Anti-p27 KIP 1 Rabbit pAb
   

Anti-p27 KIP 1 Rabbit pAb

Antibodies Oncology
AO-03-GB11154-50
CDKN4,P27KIP1,p 27,KIP1,GB11154,pK 1,cyclin-dependent kinase inhibitor 1B,pK1,GB11154100,11154,p27-KIP-1,MEN1B,pK-1,p27KIP1,MEN4,cyclin dependent kinase inhibitor 1B,KIP-1,p27 KIP 1,CDKN1B,p-27,Cyclin-dependent kinase inhibitor 1B,p27Kip1,Cdki1b,p27,Cyclin-dependent kinase inhibitor p27,P27-like cyclin-dependent kinase inhibitor
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 1B

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cyclin-dependent kinase inhibitor 1B, cyclin dependent kinase inhibitor 1B,p27Kip1, Cdki1b, p27, Cyclin-dependent kinase inhibitor p27, P27-like cyclin-dependent kinase inhibitor

Uniprot ID

P46414

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse p27 KIP1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm, Endosome



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 100-1: 500 colon, heart, hepatitis
ICC/IF Human, Mouse 1: 200-1: 800 HeLa



Background

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.



Images

0176o7U6FMbd3Mk.jpg

 IHC analysis of p27 KIP 1(GB11154).
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02Ptz7pObESEC7q.jpg

 IHC analysis of p27 KIP 1(GB11154).
Sample: Human subcutaneous transplant tumor, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03y6qyIjCcFWdEz.jpg

 IHC analysis of p27 KIP 1(GB11154).
Sample: Rat Skeletal muscle, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04TUwjpr6OMpyI1.jpg

 IF analysis of p27 KIP 1(GB11154)red.
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

058AbKuPSVkizdm.jpg

 IF analysis of p27 KIP 1(GB11154)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06p3HmJH8vHLl3Y.jpg

 IF analysis of p27 KIP 1(GB11154)red.
Sample: Rat hepatitis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07Cewyo2OqhcbHM.jpg

 IF analysis of p27 KIP 1(GB11154)red.
Sample: Rat nephritis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08rOcBENnqCW9oK.jpg

 IF analysis of p27 KIP 1(GB11154)red.
Sample: Rat pneumonia, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09h6kPvjmWy77rR.jpg

 ICC analysis of p27 KIP 1(GB11154)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11154-50
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Hurry! only 10 items left in stock.

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