Product Information
|
Protein full name |
Leucine-rich repeat and WD repeat-containing protein 1 |
|
Synonyms |
CENP-33, DKFZp434K1815, ORCA |
|
Uniprot ID |
Q8BUI3 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse LRWD1 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
72 kDa / 90 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 1000-1: 2000 | MCF7,Molt-4 |
Background
Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation (By similarity). Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
Images
|
|
WB analysis of LRWD1(GB115193). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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