Product Information
|
Protein full name |
Kelch-like protein 10 |
|
Synonyms |
kelch like 10 (Drosophila), Kelch like protein 10, KLHL10 |
|
Uniprot ID |
Q9D5V2 |
|
Immunogen |
Recombinant protein corresponding to Mouse KLHL10 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
69 kDa / 65 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 1000-1: 2000 | heart |
| ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
May be a substrate-specific adapter of a CUL3-based E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins during spermatogenesis. Required for male fertility.
Images
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WB analysis of KLHL10(GB114500). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
ICC analysis of KLHL10(GB114500)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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