Anti-Acrosin Rabbit pAb

Anti-Acrosin Rabbit pAb

Antibodies Oncology
AO-03-GB114075-100
Acrosin heavy chain,Proacrosin,Acrosin precursor,ACRS,Acrosin,GB114075,Preproacrosin,114075,Acr,GB114075100,Acrosin
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Product Information


Protein full name

Acrosin

Synonyms

Acr, Acrosin heavy chain, Acrosin precursor, ACRS, Preproacrosin, Proacrosin

Uniprot ID

P23578

Immunogen

Recombinant protein corresponding to Mouse Acrosin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 1000-1: 2000 testis



Background

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.



Images

01KPHIyROD3LqCa.jpg

 IHC analysis of Acrosin(GB114075).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02lyMntlIqFgKDX.jpg

 IF analysis of Acrosin(GB114075)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

03mKLr4IJDvXYIU.jpg

 IF analysis of Acrosin(GB114075)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114075-100
: 10 Items
Hurry! only 10 items left in stock.

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