Anti-PSME3 Rabbit pAb
   

Anti-PSME3 Rabbit pAb

Antibodies Oncology
AO-03-GB113794-100
Ki nuclear autoantigen,PA28G,PSME-3,GB113794100,GB113794,PA28 gamma,PSME3,113794,Activator of multicatalytic protease subunit 3,PSME 3,REG GAMMA,Ki,11S regulator complex subunit gamma,PA28gamma,Proteasome activator complex subunit 3
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Product Information


Protein full name

Proteasome activator complex subunit 3

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA, Activator of multicatalytic protease subunit 3, 11S regulator complex subunit gamma

Uniprot ID

P61290

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PSME3

Isotype

IgG

Predicted MW./Observed MW.

30 kDa / 30 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Proteasome



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 1000-1: 2000 MCF-7, PC-12, HeLa
IHC/IF Human, Mouse 1: 2000-1: 4000 colon, kidney, brain
ICC/IF Human, Mouse 1: 100-1: 300 HeLa, NIH/3T3



Background

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.



Images

01bSkYcikHUkNYy.jpg

 WB analysis of PSME3(GB113794).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02ArOSYILKOt3Ma.jpg

 IHC analysis of PSME3(GB113794).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03bXikfXlcat3rL.jpg

 IHC analysis of PSME3(GB113794).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04KMuwvMV2LhrKN.jpg

 IHC analysis of PSME3(GB113794).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05biZ1Lpt5QLxju.jpg

 IHC analysis of PSME3(GB113794).
Sample: Mouse liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06tgrgfgbJSDPyg.jpg

 ICC analysis of PSME3(GB113794)green.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.

07kj20Q2faTS0qJ.jpg

 ICC analysis of PSME3(GB113794)green.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113794-100
: 10 Items
Hurry! only 10 items left in stock.

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