Product Information
|
Protein full name |
Mitochondrial tRNA methylthiotransferase CDK5RAP1 |
|
Synonyms |
C20orf34, C42, CDK5RAP1, CDK5RAP1.3, CDK5RAP1.4, CGI 05, HSPC167, CDK5 activator-binding protein C42, CDK5 regulatory subunit-associated protein 1, RP5-1187J4.1 |
|
Uniprot ID |
Q8BTW8 |
|
Immunogen |
Recombinant protein corresponding to Mouse CDK5RAP1 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
66 kDa / 72 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Rat | 1: 500-1: 800 | HeLa, C6, A549 |
Background
Cyclin dependent kinase 5 (Cdk5) is a key regulator of cell cycle progression in neuronal differentiation that physically associates with and is activated by the neuron-specific protein p35. CDK5RAP1 (Cdk5 regulatory subunit-associated protein 1), also known as Cdk5 activator-binding protein C42, is a 601 amino acid protein that specifically inhibits Cdk5 activation by p35 through formation of a dimer that inhibits kinase activity.
Images
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WB analysis of CDK5RAP1(GB113634). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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