Anti-p27 KIP 1 Rabbit pAb

Anti-p27 KIP 1 Rabbit pAb

Antibodies Oncology
AO-03-GB112176-100
GB112176100,CDKN4,p 27,p27Kip1,KIP1,Cyclin-dependent kinase inhibitor p27,Cdki1b,pK 1,pK1,p27,p27-KIP-1,MEN1B,P27-like cyclin-dependent kinase inhibitor,pK-1,GB112176,p27KIP1,MEN4,KIP-1,112176,p27 KIP 1,p-27,Cyclin-dependent kinase inhibitor 1B,CDKN1B,P27KIP1,cyclin-dependent kinase inhibitor 1B,cyclin dependent kinase inhibitor 1B
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 1B

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cyclin-dependent kinase inhibitor 1B, cyclin dependent kinase inhibitor 1B,p27Kip1, Cdki1b, p27, Cyclin-dependent kinase inhibitor p27, P27-like cyclin-dependent kinase inhibitor

Uniprot ID

P46527, P46414

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse p27 KIP 1

Isotype

IgG

Predicted MW./Observed MW.

22 kDa / 27 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 heart, spleen, brain



Background

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.



Images

01TNJf8e4uBu84C.jpg

 WB analysis of p27 KIP 1(GB112176).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02a7ThJCEWrP8Oi.jpg

 WB analysis of p27 KIP 1(GB112176).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112176-100
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