Anti-CDK2 Rabbit pAb
   

Anti-CDK2 Rabbit pAb

Antibodies Oncology
AO-03-GB112129-100
p33 protein kinase,p33(CDK2),CDC28,CDKN2,Cdk-2,GB112129,CDC2A,MPF,Cdk2,Anti- Cdk2 Rabbit pAb,CDK2,Cdc2 related protein kinase,112129,GB112129100,Cdk 2,Cell division protein kinase 2,Cyclin-dependent kinase 2
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Product Information


Protein full name

Cyclin-dependent kinase 2

Synonyms

Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, CDC28, CDC2A, Cdc2 related protein kinase, p33(CDK2), MPF

Uniprot ID

P24941

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Cdk2

Isotype

IgG

Predicted MW./Observed MW.

34 kDa / 34 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 400-1: 800spleen, thymus, lymph node
IHC/IFHuman, Mouse, Rat1: 200-1: 800B lymphocytoma, spleen



Background


The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).



Images

01zFXXJ340HVCNu.jpg                        

WB analysis of CDK2(GB112129).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02u0xgUtCYATjUk.jpg                        

WB analysis of CDK2(GB112129).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03zwFGfoq9JXMNL.jpg                        

IHC analysis of CDK2(GB112129).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer  (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 350, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04CfMICXaa23ONP.jpg                        

IHC analysis of CDK2(GB112129).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer  (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 350, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05B0tDtQdWyfFAu.jpg                        

IHC analysis of CDK2(GB112129).
Sample: Human B lymphocytoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer  (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 350, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112129-100
: 10 Items
Hurry! only 10 items left in stock.

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