Anti-Cdc27 Rabbit pAb

Anti-Cdc27 Rabbit pAb

Antibodies Oncology
AO-03-GB111996-100
Cell Division Cycle 27,HNUC,Anti- Cdc27 Rabbit pAb,Cdc27,Nuc2 homolog,APC3,ANAPC3,Cdc-27,Anaphase-promoting complex subunit 3,GB111996100,GB111996,111996,Cdc 27,Cell division cycle protein 27 homolog
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Product Information


Protein full name

Cell division cycle protein 27 homolog

Synonyms

Cdc27, ANAPC3, APC3, Nuc2 homolog, HNUC, Cell Division Cycle 27, Anaphase-promoting complex subunit 3

Uniprot ID

A2A6Q5

Immunogen

Recombinant protein corresponding to Mouse Cdc27

Isotype

IgG

Predicted MW./Observed MW.

92 kDa / 92 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 K562, HeLa, Jurkat



Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.



Images

01paiQkCqNLbKp5.jpg

 WB analysis of Cdc27(GB111996).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111996-100
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