Anti-TTK/Mps1 Rabbit pAb
   

Anti-TTK/Mps1 Rabbit pAb

Antibodies Oncology
AO-03-GB111371-100
TTK/Mps1,TTK,PYT,MPS1L1,Anti-TTK/Mps1 Rabbit pAb,111371,Mps 1,MPS1,Phosphotyrosine picked threonine-protein kinase,Mps-1,GB111371100,GB111371,Dual specificity protein kinase TTK
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Product Information


Protein full name

Dual specificity protein kinase TTK

Synonyms

Phosphotyrosine picked threonine-protein kinase, PYT, TTK, MPS1, MPS1L1, ESK, TTK protein kinase

Uniprot ID

P33981

Immunogen

KLH conjugated Synthetic peptide corresponding to Human TTK

Isotype

IgG

Predicted MW./Observed MW.

97 kDa / 97 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 liver, small intestine, testis
IHC/IF Human 1: 750-1: 1500 breast, colon cancer, colon



Background

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase. Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint.



Images

01GaOlwSD3O4sZn.jpg

 WB analysis of TTK/Mps1(GB111371).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02IMw8I7JI0KWZP.jpg

 IHC analysis of TTK/Mps1(GB111371).
Sample: Human esophagus cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 750, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03TqW44Hh1Rcj84.jpg

 IHC analysis of TTK/Mps1(GB111371).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 750, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04iwthOYwp6YN7o.jpg

 IHC analysis of TTK/Mps1(GB111371).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 750, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05HCr0WQOPOnrNI.jpg

 IHC analysis of TTK/Mps1(GB111371).
Sample: Human breast, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 750, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111371-100
: 10 Items
Hurry! only 10 items left in stock.

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