Anti-CDKN2A/p16INK4a Rabbit pAb
   

Anti-CDKN2A/p16INK4a Rabbit pAb

Antibodies Oncology
AO-03-GB111143-100
CDKN2A/p16INK4a,Cyclin-dependent kinase 4 inhibitor A,CDKN-2,CDKN 2,INK-4,111143,CDK4I,p-16INK-4a,p 16,INK 4,p-16,p 16INK 4a,p16-INK4a,P16ink4a,CDKN 2A,CDKN-2A,GB111143,Cdkn2a,CDKN2A,MTS1,CDKN2,Multiple tumor suppressor 1,MTS-1,p16INK4A,p16-INK4,Cyclin-dependent kinase inhibitor 2A,Anti-p16INK4a
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 2A

Synonyms

Cyclin-dependent kinase 4 inhibitor A, CDK4I, CDKN2A, MTS1, CDKN2 , Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16INK4A, p16-INK4, p16-INK4a, Cdkn2a, P16ink4a

Uniprot ID

P51480

Immunogen

Recombinant protein corresponding to Mouse CDKN2A/p16INK4a

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human 1: 500-1: 2000 breast cancer, colon, lung cancer
ICC/IF Human 1: 200-1: 800 HeLa



Background

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.



Images

01Q1wsiHufznWdP.jpg

 IHC analysis of CDKN2A/p16INK4a(GB111143).
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02ljDdV9jz5PeKD.jpg

 IHC analysis of CDKN2A/p16INK4a(GB111143).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03gAg4zczHyjJut.jpg

 IHC analysis of CDKN2A/p16INK4a(GB111143).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04zeRgNpE15ha7i.jpg

 IHC analysis of CDKN2A/p16INK4a(GB111143).
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05CczNFdVxranKD.jpg

 IF analysis of CDKN2A/p16INK4a(GB111143)red.
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06V1tSp6kmOgLza.jpg

 IF analysis of CDKN2A/p16INK4a(GB111143)red.
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07TRnvGes5LDsHs.jpg

 ICC analysis of CDKN2A/p16INK4a(GB111143)red.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111143-100
: 10 Items
Hurry! only 10 items left in stock.

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