Anti-Rad51 Mouse mAb

Anti-Rad51 Mouse mAb

Antibodies Oncology
AO-03-GB12573-100
GB12573,12573,HHsRad51,GB12573100,HsT16930,Rad51,RAD51A,BRCC5,Rad-51,RECA,Rad 51,RAD51 recombinase,RAD51,FANCR,MRMV2,DNA repair protein RAD51 homolog 1
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Product Information


Protein full name

DNA repair protein RAD51 homolog 1

Synonyms

RAD51, BRCC5, FANCR, HHsRad51, HsT16930, MRMV2, RAD51A, RECA, RAD51 recombinase

Uniprot ID

Q08297

Immunogen

Recombinant protein corresponding to Mouse Rad51

Isotype

IgG1, κ

Clone Number

5F12G1

Purity

Affinity purification

Subcellular location

Nucleus, Cytoskeleton, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 600-1: 1600 brain



Background

RAD51 is a eukaryotic gene, which is highly conserved from yeast to humans. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. In humans, RAD51 is a 339-amino acid protein that plays a major role in homologous recombination of DNA during double strand break repair. In this process, an ATP dependent DNA strand exchange takes place in which a template strand invades base-paired strands of homologous DNA molecules. RAD51 is involved in the search for homology and strand pairing stages of the process.



Images

01MGCKD0byXW0zb.jpg

 IHC analysis of Rad51(GB12573).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

02DrPeGrq14h2gV.jpg

 IHC analysis of Rad51(GB12573).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB12573-100
: 10 Items
Hurry! only 10 items left in stock.

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