Anti-14-3-3 zeta Rabbit pAb

Anti-14-3-3 zeta Rabbit pAb

Antibodies Oncology
AO-03-GB111023-100
Ywhaz,SEZ-2,KCIP-1,14-3-3 Delta,1z,1433,14-3-3 Zeta,1433zeta,1433 zeta,GB111023100,GB111023,111023,Protein kinase C inhibitor protein 1,14 3 3,14-3-3 zeta,14-3-3 protein zeta/delta
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Product Information


Protein full name

14-3-3 protein zeta/delta

Synonyms

Protein kinase C inhibitor protein 1, KCIP-1, SEZ-2, Ywhaz, 14-3-3 Delta, 14-3-3 Zeta

Uniprot ID

P63101

Immunogen

Recombinant protein corresponding to Mouse 14-3-3 zeta

Isotype

IgG

Predicted MW./Observed MW.

28 kDa / 28 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 lung, kidney, brain
IHC/IF Mouse, Rat 1: 500-1: 2000 brain



Background

YWHAZ belongs to the 14-3-3 family of proteins which are in charge for checkpoint control, apoptotic & nutrient sensing pathways as well as signal transduction by binding to phosphoserine-containing proteins. This highly conserved and ubiquitously expressed protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. It interacts with IRS1 protein,signifying a role in regulating insulin. YWHAZ function as an adapter protein involved in the regulation of a large spectrum of both general and specialized signaling pathway.



Images

01WcOkaIdyGnNUB.jpg

 WB analysis of 14-3-3 zeta(GB111023).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

025axkNZ6OOQQIv.jpg

 WB analysis of 14-3-3 zeta(GB111023).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03JhUOo7dLz9xa7.jpg

 IHC analysis of 14-3-3 zeta(GB111023).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04vCFzrEeyU8lSG.jpg

 IHC analysis of 14-3-3 zeta(GB111023).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111023-100
: 10 Items
Hurry! only 10 items left in stock.

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