Anti-Cyclin B1 Rabbit pAb
   

Anti-Cyclin B1 Rabbit pAb

Antibodies Oncology
AO-03-GB11255-100
GB11255100,Cyclin-B1,CCNB1,Cyclin B1,G2/mitotic specific cyclin B1,CB-1,CyclinB1,B-1,Anti-CyclinB1 Rabbit pAb,B 1,CCNB,GB11255,CB 1,11255,CB1,G2/mitotic-specific cyclin-B1
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Product Information


Protein full name

G2/mitotic-specific cyclin-B1

Synonyms

CCNB, CCNB1, Cyclin B1, G2/mitotic specific cyclin B1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB

Uniprot ID

P24860

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1

Isotype

IgG

Predicted MW./Observed MW.

48 kDa / 55 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 250-1: 500 lung
IHC/IF Human, Mouse, Rat 1: 100-1: 800 brain, lung, testis



Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.



Images

01rJgSJWk2rD24M.jpg

 WB analysis of Cyclin B1(GB11255).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02zjPO0lSymMPba.jpg

 WB analysis of Cyclin B1(GB11255).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03wiGFb2UeLHkO8.jpg

 IHC analysis of Cyclin B1(GB11255).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04dG7CZ96gj9RRF.jpg

 IHC analysis of Cyclin B1(GB11255).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

053MNz7rgR1XILa.jpg

 IHC analysis of Cyclin B1(GB11255).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06awArNTJiOJLMy.jpg

 IHC analysis of Cyclin B1(GB11255).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11255-100
: 10 Items
Hurry! only 10 items left in stock.

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