Anti-Bora Rabbit pAb
   

Anti-Bora Rabbit pAb

Antibodies Oncology
AO-03-GB11647-100
GB11647100,FLJ22624,BORA,GB11647,11647,C13orf34,Bora,Protein aurora borealis,RP11-342J4.2,Chromosome 13 open reading frame 34,HsBora,Aurora borealis,Protein aurora borealis
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Product Information


Protein full name

Protein aurora borealis

Synonyms

Aurora borealis, bora, BORA, C13orf34, Chromosome 13 open reading frame 34, FLJ22624, HsBora, Protein aurora borealis, RP11-342J4.2

Uniprot ID

Q8BS90

Immunogen

Recombinant protein corresponding to Mouse Bora

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 100-1: 400 cerebellum, heart, lung



Background

Aurora related kinase-1 (ARK-1) is a centrosome-associated serine/threonine kinase that regulates centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. Bora (protein aurora borealis) is a 559 amino acid protein that activates ARK-1. Bora is localized to the nucleus until mitosis is initiated, when it then translocates to the cytoplasm. This translocation is dependent on activated Cdc2, which releases Bora to bind and activate ARK-1 in the cytoplasm.



Images

01AZ2SlcIo34mgf.jpg

 IHC analysis of Bora(GB11647).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02I3xDljEjDdsli.jpg

 IHC analysis of Bora(GB11647).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

038vyDWL6m4Vqg5.jpg

 IF analysis of Bora(GB11647)red.
Sample: Mouse epencephalon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04ATgVlyQovGySI.jpg

 IF analysis of Bora(GB11647)red.
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05XAh6XaucvisAm.jpg

 IF analysis of Bora(GB11647)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11647-100
: 10 Items
Hurry! only 10 items left in stock.

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