Product Information
Protein full name | Mitotic spindle assembly checkpoint protein MAD1 |
Synonyms | MAD1L1, PIG9, TP53I9, TXBP181, MAD1, MAD1 mitotic arrest deficient like 1, Mitotic arrest deficient 1 like 1 |
Uniprot ID | Q9WTX8 |
Immunogen | Recombinant protein corresponding to Mouse MAD1 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus, Cytoskeleton |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse | 1: 500-1: 3000 | brain |
Background
Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.
Images
| IHC analysis of MAD1(GB11644). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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