Anti-Chk1 Rabbit pAb

Anti-Chk1 Rabbit pAb

Antibodies Oncology
AO-03-GB11633-100
Chk 1,checkpoint kinase 1,11633,GB11633100,GB11633,Chk1,Chk-1,CHEK1,Serine/ threonine-protein kinase Chk1
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Product Information


Protein full name

Serine/ threonine-protein kinase Chk1

Synonyms

CHK1 checkpoint homolog, Checkpoint kinase-1, Chek1, Chk1, CHEK1, CHK1, checkpoint kinase 1

Uniprot ID

O35280

Immunogen

Recombinant protein corresponding to Mouse Chk1

Isotype

IgG

Predicted MW./Observed MW.

45 kDa / 45 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 spleen, lung



Background

Checkpoint kinase 1, commonly referred to as Chk1, is a serine/threonine-specific protein kinase that are involved in cell cycle control. Two checkpoint kinase subtypes have been identified, Chk1 and Chk2. Chk1 is a central component of genome surveillance pathways and is a key regulator of the cell cycle and cell survival. Chk1 is required for the initiation of DNA damage checkpoints and has recently been shown to play a role in the normal (unperturbed) cell cycle.



Images

011XpoSbg4jkHfO.jpg

 WB analysis of Chk1(GB11633).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02jNUAUlbRe9GKf.jpg

 WB analysis of Chk1(GB11633).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11633-100
: 10 Items
Hurry! only 10 items left in stock.

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