Anti-14-3-3 gamma Rabbit pAb

Anti-14-3-3 gamma Rabbit pAb

Antibodies Oncology
AO-03-GB11578-100
11578,1433gamma,1433 gamma,GB11578100,YWHAG,tyrosine 3-monooxygenase,14-3-3gamma,EIEE56,tryptophan 5-monooxygenase activation protein gamma,PPP1R170,1g,14-3-3 gamma,GB11578,14 3 3,1433,14-3-3 protein gamma
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Product Information


Protein full name

14-3-3 protein gamma

Synonyms

YWHAG, 14-3-3gamma, PPP1R170, tyrosine 3-monooxygenase, EIEE56, tryptophan 5-monooxygenase activation protein gamma,

Uniprot ID

P61982

Immunogen

Recombinant protein corresponding to Mouse 14-3-3 gamma

Isotype

IgG

Predicted MW./Observed MW.

28 kDa / 32 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 brain, testis, lung



Background

14-3-3 protein gamma is a protein that is encoded by the YWHAG gene. This gene product belongs to the 14-3-3 protein family which mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s.



Images

01SFd5vsDLo53Q9.jpg

 WB analysis of 14-3-3 gamma(GB11578).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02fQvsr5ayqGSj6.jpg

 WB analysis of 14-3-3 gamma(GB11578).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03PJSb2YIrE1R2H.jpg

 WB analysis of 14-3-3 gamma(GB11578).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11578-100
: 10 Items
Hurry! only 10 items left in stock.

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