Product Information
|
Protein full name |
Serine/ threonine-protein kinase Chk2 |
|
Synonyms |
CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Checkpoint kinase 2 |
|
Uniprot ID |
Q9Z265 |
|
Immunogen |
Recombinant protein corresponding to Mouse Chk2 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
61 kDa / 61 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, colon, thymus |
Background
CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 regulates cell division, and has the ability to prevent cells from dividing too rapidly or in an uncontrolled manner, So CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2.
Images
|
|
WB analysis of Chk2(GB11574). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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