Anti-Cdc42 Rabbit pAb

Anti-Cdc42 Rabbit pAb

Antibodies Oncology
AO-03-GB11570-100
GB11570100,CDC42,CDC-42,Growth regulating protein,GTP binding protein 25kD,TKS,11570,GB11570,Small GTP binding protein CDC42,CDC42Hs,G25K,CDC 42,Cell division cycle 42,G25K GTP binding protein,Cell division control protein 42 homolog
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Product Information


Protein full name

Cell division control protein 42 homolog

Synonyms

CDC42, CDC42Hs, G25K, TKS, Cell division cycle 42, G25K, G25K GTP binding protein, Growth regulating protein, GTP binding protein 25kD, Small GTP binding protein CDC42

Uniprot ID

P60766

Immunogen

Recombinant protein corresponding to Mouse CDC42

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane, Cytoskeleton



Applications


ApplicationsSpeciesDilutionPositive Tissue
IHC/IFHuman1: 500-1: 3000liver, liver cancer, stomach
ICC/IFMouse1: 50-1: 200NIH-3T3



Background


Cell division control protein 42 homolog, also known as Cdc42, is a protein involved in regulation of the cell cycle. Human Cdc42 is a small GTPase of the Rho family, which regulates signaling pathways that control diverse cellular functions including cell morphology, cell migration, endocytosis and cell cycle progression. Rho GTPases are central to dynamic actin cytoskeletal assembly and rearrangement that are the basis of cell-cell adhesion and migration.



Images

01nQTcotfbkgNbT.jpg                        

IHC analysis of Cdc42(GB11570).
Sample: Human liver, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02llKqVJhA9DNlv.jpg                        

IHC analysis of Cdc42(GB11570).
Sample: Human stomach, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03GCLHGGlgIqC58.jpg                        

IF analysis of Cdc42(GB11570)red.
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04lMUAhEwKWMSoq.jpg                        

ICC analysis of Cdc42(GB11570)red.
Sample: NIH-3T3, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 50, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11570-100
: 10 Items
Hurry! only 10 items left in stock.

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