Product Information
|
Protein full name |
Bromodeoxyuridine |
|
Synonyms |
Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse BrdU |
|
Isotype |
IgG1, κ |
|
Clone Number |
4B6C12 |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Species independent | 1: 100-1: 500 | jejunum, neonatal mouse's brain |
Background
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.
Images
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IHC analysis of BrdU(GB12051). Sample: Mouse jejunum, 4% PFA (G1101) 12-24h. Antigen retrieval: use 2M HCL in pure water for 30 minutes at 37℃ to denature the DNA. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of BrdU(GB12051). Sample: Mouse jejunum, 4% PFA (G1101) 12-24h. Antigen retrieval: use 2M HCL in pure water for 30 minutes at 37℃ to denature the DNA. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IF analysis of BrdU(GB12051)red. Sample: Mouse neonatal mouse's brain, 4% PFA (G1101) 12-24h. Antigen retrieval: use 2M HCL in pure water for 30 minutes at 37℃ to denature the DNA. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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