Anti-Cdc25 Rabbit pAb

Anti-Cdc25 Rabbit pAb

Antibodies Oncology
AO-03-GB11856-100
GB11856,CDC-25,CDC 25,H-GRF55,11856,CDC25,Ras protein specific guanine nucleotide releasing factor 1,GRF55,RASGRF1,CDC25L,GB11856100,GNRP,PP13187,Ras-GRF1,GRF1,Ras-specific guanine nucleotide-releasing factor 1
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Product Information


Protein full name

Ras-specific guanine nucleotide-releasing factor 1

Synonyms

RASGRF1, CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187, Ras-GRF1, Ras protein specific guanine nucleotide releasing factor 1

Uniprot ID

P27671

Immunogen

Recombinant protein corresponding to Mouse CDC25

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane, Cytoplasm



Applications


ApplicationsSpeciesDilutionPositive Tissue
IHC/IFMouse, Rat1: 1000-1: 4000brain



Background


The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit.



Images

01WF2CdwB3leOJY.jpg                        

IHC analysis of Cdc25(GB11856).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02mx7D3IdE1QfeK.jpg                        

IHC analysis of Cdc25(GB11856).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03zOK3JUMtNzGhC.jpg                        

IF analysis of Cdc25(GB11856)red.
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04gP038EUMkDtQL.jpg                        

IF analysis of Cdc25(GB11856)red.
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11856-100
: 10 Items
Hurry! only 10 items left in stock.

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