Product Information
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Protein full name |
Cyclin-dependent kinase 5 activator 2 |
|
Synonyms |
Cdk5r2, CDK5R2, NCK5AI, P39, p39nck5ai, cyclin-dependent kinase 5, regulatory subunit 2 (p39), cyclin dependent kinase 5 regulatory subunit 2 |
|
Uniprot ID |
O35926 |
|
Immunogen |
Recombinant protein corresponding to Mouse p39 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
39 kDa / 43 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 500-1: 1000 | brain, hippocampus, cerebral cortex |
| IHC/IF | Mouse, Rat | 1: 750-1: 3000 | cerebellum |
Background
Cyclin-dependent kinase 5 activator 2 is an enzyme encoded by the CDK5R2 gene. The protein is a neuron-specific activator of CDK5 kinase. It associates with CDK5 to form an active kinase. This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins.
Images
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WB analysis of p39(GB11792). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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IHC analysis of p39(GB11792). Sample: Mouse epencephalon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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