Product Information
Protein full name | Toll-like receptor 4 |
Synonyms | Lps,TLR4, ARMD10, CD284, TLR-4, TOLL, toll like receptor 4 |
Uniprot ID | Q9QUK6 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse TLR4 |
Predicted MW./Observed MW. | 95 kDa / 95 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | THP-1, RAW264.7, thymus |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-TLR4 antibody(ZB11519-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
TLR4 is a transmembrane protein, member of the toll-like receptor family, which belongs to the pattern recognition receptor (PRR) family. Its activation leads to an intracellular signaling pathway NF-κB and inflammatory cytokine production which is responsible for activating the innate immune system.It is most well known for recognizinglipopolysaccharide (LPS), a component present in many Gram-negative bacteria (e.g. Neisseria spp.) and selectGram-positive bacteria. Its ligands also include several viral proteins, polysaccharide, and a variety of endogenous proteins such as low-density lipoprotein, beta-defensins, and heat shock protein.
Images
| WB analysis of TLR4(ZB11519-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-TLR4 antibody 1: 500, 4℃ overnight. |
| WB analysis of TLR4(ZB11519-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-TLR4 antibody 1: 500, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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