Anti-TNF-alpha antibody (HRP Conjugated)

Anti-TNF-alpha antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB115726-HRP-50
TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor,Tumour necrosis factor alpha,tumor necrosis factor,TNLG1,TNF-α,TNF a,TNF alpha,NFα,TNF-α,Cachectin,TNFα
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Product Information


Protein full name

Tumor necrosis factor alpha

Synonyms

TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor, Tumour necrosis factor alpha,tumor necrosis factor, TNLG1, TNF-α, TNF a, TNF alpha,NFα, TNF-α, Cachectin, TNFα

Uniprot ID

P01375

Immunogen

Recombinant protein corresponding to Human TNF-alpha

Predicted MW./Observed MW.

26 kDa / 25/28 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Mouse 1: 500-1: 1000 RAW264.7
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-TNF-alpha antibody(ZB115726-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.



Images

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 WB analysis of TNF-alpha(ZB115726-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-TNF-alpha antibody 1: 500, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB115726-HRP-50
: 10 Items
Hurry! only 10 items left in stock.

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