Anti-p21 antibody (HRP Conjugated)

Anti-p21 antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB115313-HRP-50
CDKN1A,CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1,p21,cyclin-dependent kinase inhibitor 1A,cyclin dependent kinase inhibitor 1A
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Product Information


Protein full name

Cyclin-dependent kinase inhibitor 1

Synonyms

CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, p21, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase inhibitor 1A

Uniprot ID

P39689

Immunogen

Recombinant protein corresponding to Mouse p21

Predicted MW./Observed MW.

19 kDa / 20 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 300-1: 600 293, MCF7, T-47D
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-p21 antibody(ZB115313-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.



Images

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 WB analysis of p21(ZB115313-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-p21 antibody 1: 300, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB115313-HRP-50
: 10 Items
Hurry! only 10 items left in stock.

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