Anti-GSK3 beta antibody (HRP Conjugated)

Anti-GSK3 beta antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB12835-HRP-50
GSK3B,Gsk3b,7330414F15Rik,8430431H08Rik,C86142,GSK-3,GSK-3beta,GSK3,glycogen synthase kinase 3 beta
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Product Information


Protein full name

Glycogen synthase kinase-3 beta

Synonyms

GSK3B, Gsk3b, 7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3, glycogen synthase kinase 3 beta

Uniprot ID

Q9WV60

Immunogen

Recombinant protein corresponding to Mouse GSK3 beta

Predicted MW./Observed MW.

47 kDa / 47 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 HUVEC, brain
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-GSK3 beta antibody(ZB12835-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background

Glycogen synthase kinase-3 (GSK-3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and an inactivating agent of glycogen synthase. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. It might be a new therapeutic target for ischemic stroke.



Images

011WhmLjy49irH9.jpg

 WB analysis of GSK3 beta(ZB12835-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-GSK3 beta antibody 1: 500, 4℃ overnight.

02QBsL6K8ZblqDM.jpg

 WB analysis of GSK3 beta(ZB12835-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-GSK3 beta antibody 1: 500, 4℃ overnight.

03vK4AlMyOQBADz.jpg

 WB analysis of GSK3 beta(ZB12835-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-GSK3 beta antibody 1: 500, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB12835-HRP-50
: 10 Items
Hurry! only 10 items left in stock.

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