Product Information
Protein full name | Cyclin-dependent kinase inhibitor 1 |
Synonyms | CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, p21, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase inhibitor 1A |
Uniprot ID | P39689 |
Immunogen | Recombinant protein corresponding to Mouse p21 |
Predicted MW./Observed MW. | 19 kDa / 20 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 300-1: 600 | 293, MCF7, T-47D |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-p21 antibody(ZB115313-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
Images
| WB analysis of p21(ZB115313-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-p21 antibody 1: 300, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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