Product Information
Protein full name | Lamin-B1 |
Synonyms | LMNB1, LMN2, LMNB, ADLD, LMN |
Uniprot ID | P20700 |
Immunogen | Recombinant protein corresponding to Human Lamin B1 |
Predicted MW./Observed MW. | 66 kDa / 25/45/66-70 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 | Jurkat, HeLa, C6 |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-Lamin B1 antibody(ZB151802-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane.
Images
| WB analysis of Lamin B1(ZB151802-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-Lamin B1 antibody 1: 1000, 4℃ overnight. |
| WB analysis of Lamin B1(ZB151802-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-Lamin B1 antibody 1: 1000, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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