Recombinant Anti-beta Actin antibody (HRP Conjugated)

Recombinant Anti-beta Actin antibody (HRP Conjugated)

Antibodies-Oncology
AO-03-ZB15001-HRP-100
β Actin,b Actin,βA,bA,actin beta,直标,ZB15001HRP,BRWS1,ACTB,Beta-actin,Beta Cytoskeletal Actin,PS1TP5BP1,直标内参,β-Actin,Actin,cytoplasmic 1,action,ACTIN-HRP
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Product Information


Protein full name

Actin, cytoplasmic 1

Synonyms

ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin

Uniprot ID

P60710

Predicted MW./Observed MW.

42 kDa / 42 kDa

Purity

Affinity purification

conjugation

HRP



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat, Pg, Ch, Bv, Sh, Gt, Mk, Rb, Ta, Frog1: 1000-1: 3000HeLa, HepG2, brain, etc.
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated anti-beta Actin antibody (ZB15001-HRP) for 2-3 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background


Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.



Images

01HMRowVKnAei5J.jpg                        

WB analysis of (beta Actin)ZB15001-HRP.
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-beta Actin antibody 1:3000, 4℃ overnight.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB15001-HRP-100
: 10 Items
Hurry! only 10 items left in stock.

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