Anti-NF-κB p65 antibody (HRP conjugated)

Anti-NF-κB p65 antibody (HRP conjugated)

Antibodies-Oncology
AO-03-ZB11997-HRP-50
RELA,NFKB3,p65,RELA proto-oncogene,NF-kB subunit
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Product Information


Protein full name

Transcription factor p65

Synonyms

RELA, NFKB3, p65, RELA proto-oncogene, NF-kB subunit

Uniprot ID

Q04207

Immunogen

Recombinant protein corresponding to Mouse Nuclear factor NF-kappa-B p65 subunit

Predicted MW./Observed MW.

65 kDa / 65 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 HeLa, RAW264.7, thymus
Incubation condition After blocking, incubate the membrane with HRP conjugated anti-NF-κB p65 antibody(ZB11997-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST



Background

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.



Images

01F7APvgrRzk7bY.jpg

 WB analysis of NF-κB p65(ZB11997-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-NF-κB p65 antibody 1: 500, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB11997-HRP-50
: 10 Items
Hurry! only 10 items left in stock.

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