Anti-Collagen I antibody (HRP conjugated)

Antibodies-Oncology
AO-03-ZB115707-HRP-50
COL1A1,EDSC,OI1,OI2,OI3,OI4,collagen type I alpha 1,collagen type I alpha 1 chain
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Product Information


Protein full name

Collagen alpha-1(I) chain

Synonyms

COL1A1, EDSC, OI1, OI2, OI3, OI4, collagen type I alpha 1, collagen type I alpha 1 chain

Uniprot ID

P02452

Immunogen

KLH conjugated Synthetic peptide corresponding to Human COL1A1

Predicted MW./Observed MW.

139 kDa / 120-140 kDa

Purity

Affinity purification

conjugation

HRP



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 skin
Incubation condition After blocking, incubate the membrane with HRP conjugated Anti-Collagen I antibody(ZB115707-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST



Background

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.



Images

015IkBEGTfqV69T.jpg

 WB analysis of Collagen I(ZB115707-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-Collagen I antibody 1: 500, 4℃ overnight.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB115707-HRP-50
: 10 Items
Hurry! only 10 items left in stock.

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