Product Information
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Protein full name
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alpha smooth muscle Actin
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Synonyms
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Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46, SMA, a-sma, sma
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Uniprot ID
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P62737
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Immunogen
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KLH conjugated Synthetic peptide corresponding to Mouse Sarcomeric Alpha Actinin
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Predicted MW./Observed MW.
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42 kDa / 42 kDa
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Purity
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Affinity purification
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conjugation
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HRP
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Applications
| Applications |
Species |
Dilution |
Positive Tissue |
| WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
C2C12, liver, RAW264.7 |
Background
Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation.
Images
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WB analysis of alpha smooth muscle Actin(ZB111364-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-alpha smooth muscle Actin antibody 1: 1000, 4℃ overnight.
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Storage
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Storage
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Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
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Storage Buffer
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PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.
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NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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