Anti-TATA binding protein TBP antibody (HRP conjugated)

Anti-TATA binding protein TBP antibody (HRP conjugated)

Antibodies-Oncology
AO-03-ZB113810-HRP-100
GTF2D,GTF2D1,SCA17,TATA-binding factor,TATA-box factor,TATA sequence binding protein,TBP,TF2D,TFIID,TATA-box-binding protein
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Product Information


Protein full name

TATA-box-binding protein

Synonyms

GTF2D, GTF2D1, SCA17, TATA-binding factor, TATA-box factor, TATA sequence binding protein, TBP, TF2D, TFIID

Uniprot ID

P29037

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse TBP

Predicted MW./Observed MW.

35 kDa / 35 kDa

Purity

Affinity purification

conjugation

HRP



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBMouse, Rat1: 200-1: 500testis
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated Anti-TATA binding protein TBP antibody (ZB113810-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background


The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene.



Images

01gZnf4jg2XtSlU.jpg                        

WB analysis of TATA binding protein TBP(ZB113810-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-TATA binding protein TBP antibody 1: 500, 4℃ overnight.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB113810-HRP-100
: 10 Items
Hurry! only 10 items left in stock.

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