Product Information
Protein full name | Cytochrome c oxidase subunit 6C |
Synonyms | Cytochrome c oxidase polypeptide VIc, Cox6c, EC 1.9.3.1 |
Uniprot ID | P09669 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human COX6C |
Predicted MW./Observed MW. | 9 kDa / 12 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 250-1: 1000 | heart |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-Cytochrome C Oxidase subunit VIc/COX6C antibody (ZB111489-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
COX6C(Cytochrome c oxidase subunit 6C) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase (subunit VIc), the terminal oxidase in mitochondrial electron transport.Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex.
Images
| WB analysis of Cytochrome C Oxidase subunit VIc/COX6C(ZB111489-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-Cytochrome C Oxidase subunit VIc/COX6C antibody 1: 1000, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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