Product Information
Protein full name | V5 tag |
Synonyms | GKPIPNPLLGLDST, V5, V5 tag, V5-tag |
Immunogen | KLH conjugated Synthetic peptide corresponding to V5 tag |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Species independent | 1: 500-1: 1000 | V5-Tag fusion portein |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Recombinant Anti-V5 tag antibody (ZB15019-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
The V5 Tag Peptide is a synthetic peptide consisting of 14 amino acids isolated from the P and V proteins of simian virus 5(SV5).The antibody is developed against the V5 Tag peptide , which presents in many vectors.
Images
| WB analysis of V5 tag(ZB15019-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-V5 tag antibody 1: 1000, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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