Product Information
Protein full name | GST Tag fusion protein |
Synonyms | Glutathione S-Transferase, GST, gst tag |
Immunogen | Recombinant GST protein |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Species independent | 1: 1000-1: 3000 | The GST-Tag fusion protein |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-GST Tag antibody(ZB15085-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
Images
| WB analysis of GST Tag(ZB15085-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-GST Tag antibody 1: 3000, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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