Recombinant Anti-Cardiac Troponin T antibody (Rabbit mAb)

Protein full name

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle, LVNC6, troponin T2, cardiac type

Immunogen

Recombinant protein corresponding to Mouse Cardiac Troponin T

Uniprot ID

P45379

Isotype

IgG

Clone Number

SB742

Purity

Affinity purification

Subcellular location

myofibril, sarcomere, sarcoplasm, striated muscle thin filament

Predicted MW./Observed MW.

36 kDa / 40 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 15000-1: 75000

heart

IHC/IF

Human, Mouse, Rat, Monkey

1: 800-1: 2400

heart

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Images

WB analysis of Cardiac Troponin T(GB153806).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 15000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Cardiac Troponin T(GB153806).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of Cardiac Troponin T(GB153806)red.
Sample Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.