Recombinant Anti-ALPP/ALPPL2/ALPI antibody (Rabbit mAb)

Protein full name

Alkaline phosphatase

Synonyms

ALPP, ALPPL2, Alkaline phosphatase, Alpp, Placental alkaline phosphatase 1, Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type, ALPPL2, ALPG, Placental alkaline phosphatase-like (PLAP-like), Germ cell alkaline phosphatase, Alkaline phosphatase, placental-like, Alkaline phosphatase Nagao isozyme, ALP-1, ALP-1, Alpi, Intestinal Alkaline phosphatase, IAP

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Alkaline phosphatase

Uniprot ID

F8VPQ6

Isotype

IgG

Clone Number

SB751

Purity

Affinity purification

Subcellular location

Cell membrane

Predicted MW./Observed MW.

60 kDa / 62-72 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 1000-1: 2000

Caco-2, A-431

IHC/IF

Human

1: 400-1: 1200

placenta, small intestine

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.

Images

WB analysis of ALPP/ALPPL2/ALPI(GB150243).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of ALPP/ALPPL2/ALPI(GB150243).
Sample: Human small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of ALPP/ALPPL2/ALPI(GB150243).
Sample: Human placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1200, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.