Recombinant Anti-p40-DeltaNp63 antibody (Rabbit mAb)

Protein full name

Tumor protein 63

Synonyms

AIS, B(p51A), B(p51B), CUSP, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, tumor protein p63, Tumor protein p73 like

Immunogen

KLH conjugated Synthetic peptide corresponding to Human P40

Uniprot ID

Q9H3D4

Isotype

IgG, κ

Clone Number

SB53

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW./Observed MW.

76 kDa / 76 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 2000

A-431

IHC/IF

Human

1: 500-1: 2000

esophageal carcinoma

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Images

WB analysis of p40-DeltaNp63(GB150003).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of p40-DeltaNp63(GB150003).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.