Recombinant Anti-CDX2 antibody (Mouse mAb)

Protein full name

Homeobox protein CDX-2

Synonyms

caudal type homeobox 2, Caudal type homeobox protein 2, CDX 3, CDX2, CDX-2, CDX3, Homeobox protein CDX 2

Immunogen

KLH conjugated Synthetic peptide corresponding to Human CDX2

Uniprot ID

Q99626

Isotype

IgG1, κ

Clone Number

SB30

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW./Observed MW.

34 kDa / 38 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 2000

Caco-2

IHC/IF

Human, Mouse, Rat

1: 500-1: 2000

colon cancer, colon

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis.

Images

WB analysis of CDX2(GB151501).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

IHC analysis of CDX2(GB151501).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of CDX2(GB151501).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.