Recombinant Anti-GATA3 antibody (Mouse mAb)

Protein full name

Trans-acting T-cell-specific transcription factor GATA-3

Synonyms

GATA3, GATA-binding factor 3, Trans acting T cell specific transcription factor GATA 3, GATA3_HUMAN, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3

Immunogen

Recombinant protein corresponding to Human GATA-3

Uniprot ID

P23771

Isotype

IgG2B

Clone Number

SB713

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW./Observed MW.

48 kDa / 48 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 2000

JurKat, EL4, thymus

IHC/IF

Human, Mouse, Rat

1: 1000-1: 3000

urinary bladder, skin, breast cancer

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression.Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.

Images

WB analysis of GATA3(GB150220).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

IHC analysis of GATA3(GB150220).
Sample: Human breast cancert, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

IF analysis of GATA3(GB150220)red.
Sample: Rat skin, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.